環境中常見的銅是生物代謝所需的微量元素，然而過量的銅會引起氧化壓力對細胞造成損傷，例如引起脂質過氧化反應。銅污染會造成對石珊瑚的生存威脅，即使曝露在稍高於背景的濃度，就會造成珊瑚共生藻密度下降及生長抑制。為瞭解在氧化壓力下珊瑚細胞膜脂質的調節適應，本研究在96小時內進行鈍枝列孔珊瑚( Seriatopora caliendrum )銅曝露實驗，處理組添加濃度為1-5 μg Cu/L。分析珊瑚組織及其共生藻細胞的甘油磷脂醯膽鹼組成，發現隨著銅曝露濃度增加，脂質組成改變伴隨珊瑚的生理變化。基於這些改變的脂質的物理化學性質，可以說明珊瑚細胞膜的調適與銅引起的氧化壓力的關聯性。

Copper, a common element in the ambient, is an essential microelement of metabolic system in the organisms. However, cells can be injured, such as the membrane lipid peroxidation, while inducing oxidative stress by exposing to excess copper levels. Copper pollution was shown to be a threat to the subsistence of scleractinian corals, even a slight increase in background levels causing the loss of symbiotic algae and the inhibition of growth. To gain insight into the accommodation of the coral cellular membrane to oxidative conditions, the coral Seriatopora caliendrum was exposed to copper levels above the background by 1-5 μg Cu/L within the 96-h duration. Glycerolphosphocholine profiling of full coral tissue and the symbiotic algae were characterized. The alteration of lipid profile accompanying several physiological changes was obtained in the coral exposed to the increased copper levels. Based on the physicochemical properties of these changed lipids, the relevance of the lipid metabolism to the accommodation of cellular membrane to copper-induced oxidative situation was explained in the coral.

第一章 前言 1
1-1 海洋污染與珊瑚白化問題 1
1-2 珊瑚與共生藻的共生關係 3
1-3 銅對生物的影響 4
1-4 脂質體學簡介 7
1-5 研究目的 10
第二章 材料與方法 11
2-1珊瑚銅暴露實驗架構 11
2-2 銅劑量分析 12
2-3 葉綠素螢光參數測量 12
2-4 脂質分析 13
2-4-1 珊瑚樣本前處理 13
2-4-2 樣本脂質萃取 14
2-4-3 高效液相層析串聯質譜儀分析 14
2-4-4數據處理與統計分析 15
第三章 結果 17
3-1 水質參數 17
3-1-1 溶氧飽和度 18
3-1-2 pH值 18
3-2 珊瑚整體生理變化 21
3-2-1 珊瑚外觀變化 21
3-2-2 葉綠素螢光分析 23
3-3 脂質組成變化 25
3-3-1 共生藻脂質組成變化 25
(一) 銅暴露劑量效應實驗 25
(二) 銅暴露時間序列實驗 27
3-3-2珊瑚總組織脂質組成變化 29
(一) 銅暴露對珊瑚總組織劑量效應之影響 29
(二) 銅暴露時間效應對珊瑚總組織之影響 30
第四章 討論 39
4-1 珊瑚整體生理變化 39
4-1-1 珊瑚分泌黏液作為逆境防護機制 39
4-1-2 銅暴露影響珊瑚光合作用與呼吸作用 40
4-2 脂質組成變化 42
4-2-1 共生藻脂質組成變化 42
(一) 提升細胞膜通透性促進ROS排出體外 42
(二) 共生藻的抗氧化壓力機制 43
4-2-2 珊瑚總組織脂質組成變化 44
(一) 降低細胞膜通透性防止ROS擴散 44
(二) 增加細胞膜張力維持結構完整 45
(三) 修復氧化壓力引起的細胞膜損傷 46
(四) 共生藻與珊瑚共享資源 47
4-3 未來研究發展 48
第五章 結論 49
參考文獻 51
英文文獻 51
中文文獻 64
網路資料 64

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中文文獻：
方天熹，2001，〈海洋污染〉，《物理雙月刊》，第23卷第3期，頁427-432。
韓柏檉，1989，〈海洋環境中銅之形態、分布、生物累積與錯合能力之研究〉，國立台灣大學海洋研究所博士論文。
網路資料：
Australian Government- Great Barrier Reef Marine Park Authority
http://www.gbrmpa.gov.au/
LIPID MAPS
http://www.lipidmaps.org/
United States Environmental Protection Agency
https://www.epa.gov/
行政院環保署
https://www.epa.gov.tw/
臺灣環境保護資訊協會-環境資訊中心
http://e-info.org.tw/