本研究使用簡易的合成技術製備出銀奈米線膠體溶液，再透過化學轉換法將塗佈於FTO的銀奈米線硫化成硫化銀奈米線，最後將此結構應用在尿酸與亞甲藍的偵測應用上。在尿酸偵測應用上，實驗中藉以電化學量測模式分析，通過循環伏安法證實硫化銀奈米線確實可檢測尿酸，再分別進行偵測極限濃度0.1 μM、濃度偵測範圍0.2-1666.5 μM、量測響應靈敏度0.04287 μAμM-1cm-2。實驗結果證明硫化銀奈米線在偵測尿酸具有一定發展潛力。
在偵測亞甲藍方面，實驗中利用了拉曼量測技術進行檢測，利用硫化銀奈米線的高表面積且與亞甲藍且有良好的吸附特性，藉由表面增強拉曼光譜進行實驗，量測亞甲藍偵測極限濃度為0.1 μM，增強因子為2.76x104，實驗結果證明硫化銀奈米線/FTO也具有一定的偵測能力。

In this study, a facile and simple method was utilized to synthesize silver nanowires (Ag NWs), and then a hydrothermal method was used to chemically convert Ag NWs to Ag2S NWs. The as-grown sample was applied to sensing the uric acid and methylene blue. As regards the electrochemical detection of uric acid, Ag2S NWs can achieve the sensitivity of 0.04287 μAμM-1cm-2 under the concentration ranging from 0.2 to 1666.5 μM with a detection limit as low as 0.1 μM. In terms of the methylene blue detection, the analytic route was based on surface-enhanced Raman spectroscopy (SERS). According to our results, the detection of limited of 0.1 μM was obtained with the enhancement factor 2.76x104.

第一章 序論 1
1.1 前言 1
1.1.1 葡萄糖簡介 2
1.1.2 尿酸簡介 2
1.1.3 亞甲藍簡介 3
1.1.4 拉曼光譜 4
1.1.5 電化學分析 5
第二章 實驗原理 6
2.1 化學分析法(electrochemical analysis) 6
2.1.1 循環伏安法(cyclic voltammetry(CV)) 6
2.1.2 安培法(Amperometric i-t curve(I-T)) 6
2.2 葡萄糖生物感測器 6
2.3 尿酸生物感測器 7
2.3.1 還原分析 (Reducing Methods) 7
2.3.2 酵素分析 (Enzymatic Methods) 7
2.4 表面增強拉曼散射(Surface Enhanced Raman Scattering) 8
2.4.1 電磁理論 9
2.4.2 化學理論 9
材料介紹 10
2.4.3 銀奈米線 10
2.4.4 硫化銀奈米線 10
2.4.5 葡萄糖 11
2.4.6 尿酸 11
2.4.7 亞甲基藍 11
第三章 實驗步驟與方法 12
3.1 奈米線合成 12
3.1.1 銀奈米線合成 12
3.2 硫化銀奈米線合成 13
3.2.1 葡萄糖及尿酸偵測 14
3.2.2 SERS量測 14
3.3 實驗儀器介紹及研究原理 15
3.3.1 掃描式電子顯微鏡(Field Emission Scanning Electron microscope (SEM)) 15
3.3.2 X射線光電子能譜儀(X-ray Photoelectron Spectrometer(XPS)) 16
3.3.3 X-射線繞射儀(X-ray scattering techniques (XRD)) 17
3.3.4 拉曼光譜(Raman spectrum) 18
3.3.5 電化學分析儀 19
第四章 結果與討論 20
4.1 硫化銀奈米線之備製及應用於電化學偵測 20
4.1.1 前言-硫化銀奈米線 20
4.1.2 FE-SEM 之形貌分析 21
4.1.3 XRD之結構分析 25
4.1.4 Raman 27
4.1.5 XPS鍵結分析 28
4.1.6 CV curve 偵測葡萄糖之分析 29
4.1.7 I-t curve偵測葡萄糖之分析 31
4.1.8 參考文獻對比 32
4.1.9 CV curve 偵測尿酸之分析 33
4.1.10 掃描速率 34
4.1.11 I-t curve偵測尿酸之分析 35
4.1.12 抗干擾能力測試 37
4.1.13 穩定性測試 38
4.1.14 參考文獻對比 39
4.1.15 結論 40
4.2 硫化銀奈米線應用於表面拉曼增益 41
4.2.1 前言-硫化銀奈米線/FTO基板SERS應用 41
4.2.2 各濃度SERS 42
4.2.3 Raman對比 43
4.2.4 SERS強度增益 44
4.2.5 增強因子 45
4.2.6 參考文獻對比 46
4.2.7 結論 46
第五章 結論與未來展望 47
引用 48

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